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An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
 

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

Article DOI: 10.3791/2094-v • 08:09 min • December 19th, 2010
December 19th, 2010 •
1, 1
1Department of Biochemistry, University of Toronto

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Resumo
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The activity of the inducible lysine decarboxylase is monitored by reacting the substrate L-lysine and the product cadaverine with 2,4,6-trinitrobenzensulfonic acid to form adducts that have differential solubility in toluene.

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Assay Measuring Activity Escherichia Coli Inducible Lysine Decarboxyase PH Acidic PH Survival Amino Acid Decarboxylases Glutamic Acid Decarboxylases Arginine Decarboxylase Lysine Decarboxylase Ornithine Decarboxylase Pyridoxal-5'-phosphate Co-factor Inner-membrane Substrate-product Antiporters Lysine-cadaverine Antiporter CadB X-ray Crystal Structure Stringent Response Regulator Guanosine 5'-diphosphate 3'-diphosphate PpGpp Stringent Response
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