Electroencephalography-Based Recording Technique to Record the Epileptiform Activity
Transcrição
Prepare the electrophysiology setup in a closed circuit. Verify that the flow rate of the interface-type chamber is set to two milliliters per minute, and open the carbox valve. Check the water level in the system, and place a piece of filter paper into the interface recording chamber to drain any excess medium.
Place a piece of lens-cleaning cleaning paper beneath the frame to supply medium to the slice, and turn on the temperature controller, amplifiers, and micromanipulators.
Use a syringe to load the glass electrode with freshly-prepared artificial cerebrospinal fluid. Place the glass electrode into the receiving electrode, and wait for the temperature in the interface chamber to stabilize at 37 degrees Celsius.
Working in a biosafety cabinet, place the insert in a 60-millimeter plate with a drop of medium. Use a sharp blade to cut a slice from the insert, and place the slice in the interface chamber with the hippocampus to the bottom right. Then, place the receiving electrode into the CA3 pyramidal cell layer.
Initiate the continuous acquisition protocol, and record the slice for 30 minutes.
