Journal
/
Biologia
/
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

DOI:
10.3791/2154-v

•

10:59 min

•

October 23, 2010

•

, , ,
1Department of Biomedical Engineering,Cornell University, 2Current Address: Mechanical Engineering Department,Dogus University

Capítulos

  • 00:00Título
  • 00:21Subscription Locl
  • 00:46Introduction
  • 01:37Preparing the Hammocks
  • 02:53 Ex ovo Culturing of the Chicken Embryos
  • 05:26Microinjection into the Vasculature
  • 08:34Left Atrial Ligation
  • 09:53Representative Results
  • 10:30Conclusion

Summary

Tadução automática

Tadução automática

In this article, we present a simple methodology to enable long-term ex-ovo avian embryo culture. This technique is ideal for longitudinal experimentation requiring complete optical accessibility and/or sterile transportation in avian embryos.

Tags

Ex-ovo Chicken Embryo Culture SystemImagingMicrosurgery ApplicationsGenetic FactorsMicroenvironmental FactorsEmbryonic DevelopmentTherapeutic StrategiesImaging TechnologyBody Plan OrganizationEmbryonic MorphogenesisChicken EmbryosVertebrate Animal Model SystemCulture And Surgical ManipulationFilter Paper RingsOptical AccessCell PatterningFate StudiesCardiac MorphogenesisEggshell Window TechniqueHigh Resolution ImagingUltrasonographyShell-less Culture SystemEnvironmental Chamber

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/2154?article_type=v

Windowing Chicken Eggs for Developmental Studies
check_url/pt/2154?article_type=v

Bioelectric Analyses of an Osseointegrated Intelligent Implant Design System for Amputees
check_url/pt/2154?article_type=v

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
check_url/pt/2154?article_type=v

Xenotransplantation of Human Stem Cells into the Chicken Embryo
check_url/pt/2154?article_type=v

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
check_url/pt/2154?article_type=v

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
check_url/pt/2154?article_type=v

Gene Transfer i ældre kyllingeembryoer af<em> Ex ovo</em> Elektroporation
check_url/pt/2154?article_type=v

Mus embryonale udvikling i et serum-frit Hele Embryo Culture System
check_url/pt/2154?article_type=v

En<em> Ex vivo</em> Kultur System til Studieinformationen Thyroid Development
check_url/pt/2154?article_type=v

Fremstilling af rotteserum Egnet til pattedyr Hele Embryo Culture

Read Article