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Capítulos
- 00:00The Method of Rodent Whole Embryo Culture Using the Rotator-type Culture System
- 00:07Introduction
- 02:06Setting up Whole Embryo Culture System
- 02:52Preparation of Culture Medium
- 03:53Removal of Embryos from the Uterus
- 04:52Dissection of Rat Embryos
- 07:27Results and Discussion
Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.
Tags
Rodent Whole Embryo CultureWEC TechniqueRotator-type Bottle Culture SystemBiologia do DesenvolvimentoMouse EmbryosRat EmbryosEx Vivo ConditionEmbryonic DayPlacenta FunctionManipulation Under MicroscopeDynamic Developmental ProcessesPostimplanted Mammalian EmbryosLong-term CultureRat WEC After E12.5Refined ModelApplications In Mammalian Developmental Biology
