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Preparation of Complaint Matrices for Quantifying Cellular Contraction
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JoVE Journal Bioengenharia
Preparation of Complaint Matrices for Quantifying Cellular Contraction

Preparation of Complaint Matrices for Quantifying Cellular Contraction

DOI:
10.3791/2173-v

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11:38 min

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December 14, 2010

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, , , ,
1Institute for Biophysical Dynamics,University of Chicago, 2Physics Department – James Franck Institute,University of Chicago, 3Interdisciplinary Scientist Training Program,University of Chicago

Capítulos

  • 00:05Título
  • 01:15Coverslip Surface Activation
  • 03:00Polyacrylamide (PAA) Gel Preparation
  • 05:07Extracellular Matrix (ECM) Protein Crosslinking to the PAA Gel
  • 07:30Confocal Imaging
  • 09:47Representative Results for Quantifying Cellular Contraction
  • 11:15Conclusion

Summary

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In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.

Tags

Complaint MatricesQuantifying Cellular ContractionCellular AdhesionExtracellular Matrix (ECM)Focal AdhesionsF-actin CytoskeletonMechanical ForcesCell MigrationECM RemodelingCell Culture SubstratesTraction Force Microscopy (TFM)Substrate DeformationsCytoskeletal OrganizationFluorescence Microscopy

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