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Protozoa Termit Gut Karşı Ligandla litik Peptitler Protozoacidal Aktivitesi Test In vitro (Protozoa Kültür) ve In vivo (Mikroenjeksiyon içine Termit Hindgut)
JoVE Journal
Biologia
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JoVE Journal Biologia
Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)
DOI:

17:36 min

December 29, 2010

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Capítulos

  • 00:05Título
  • 01:48Introduction
  • 02:51Creation and Preparation of Peptides and Lytic Peptide Conjugates
  • 03:43Extraction of Termite Gut Protozoa Under Anaerobic Conditions
  • 06:59Binding of Fluorescent-coupled Ligand to Termite Gut Protozoa
  • 08:12Binding Ligand-coupled Lytic Peptides to Termite Gut Protozoa
  • 09:07Injecting Fluorescent-coupled Ligand into the Termite Hindgut
  • 12:08Injecting Ligand-coupled Lytic Peptide into the Termite Hindgut
  • 12:59Results: Synthesized Ligand Increases Lytic Peptide Binding and Activity Against Termite Gut Protozoa
  • 15:14Conclusion

Summary

Tadução automática

Ligandlar protozoa Formosan litik peptitler ile birleştiğinde floresan mikroskopi ve bu ligandlar kullanılarak yeraltı termitler bağırsak protozoa öldürmek selüloz sindiren bu yüzey membran bağlamak gösteren prosedürleri mevcut<em> In vitro</em> (Anaerobik protozoa kültürü) ve<em> In vivo</em> (Termit hindgut içine enjeksiyon).

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