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Groei en differentiatie van volwassen hippocampus Arctic grondeekhoorn neurale stamcellen
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Capítulos
- 00:05Título
- 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
- 01:05Preparing poly-L-ornithine-coated Flasks
- 01:56Thawing and Seeding agsNSC
- 03:39Expanding agsNSC
- 04:11Trypsinization of Cells
- 05:27Inoculating Well Plates for Differentiation
- 07:25Maintenance of Neurons
- 08:09Results of Growth and Differentiation of agsNSC
- 09:29Conclusion
Neurale stamcellen werden bereid uit de hippocampus van de volwassen niet-winterslaap jaarling Arctic grondeekhoorns (AGS). Deze neurale stamcellen kan worden uitgebreid door middel van talrijke passages, gedifferentieerde en onderhouden als een bijna 50:50 neuron te glia-cultuur.
Tags
GrowthDifferentiationAdult HippocampalArctic Ground SquirrelNeural Stem CellsHibernationIschemic InjuryBrainOxygen-glucose DeprivationNeuronsProteinsGene TargetsTherapiesIschemic IndicationsCold ToleranceProliferationCultureTUJ1-positive NeuronsToxic ChemicalsNeurogenesisMEK/ERK Signaling PathwayPI3K/Akt Signaling Pathway
