Preparation of Highly Coupled Rat Heart Mitochondria

Irina Gostimskaya; Alexander Galkin

doi:10.3791/2202

Journal

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Biologia

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Preparation of Highly Coupled Rat Heart Mitochondria

JoVE Journal
Biologia
Author Produced

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JoVE Journal Biologia

Preparation of Highly Coupled Rat Heart Mitochondria

DOI:

10.3791/2202-v

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12:28 min

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September 23, 2010

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Irina Gostimskaya, Alexander Galkin

¹Faculty of Life Sciences,University of Manchester, ²School of Biological Sciences,Queen’s University Belfast

Capítulos

00:00Título
00:07Introduction
00:14General Scheme of the Protocol
12:19Conclusion

Summary

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We describe а protocol for isolation of pure, highly coupled rat heart mitochondria for functional or structural studies of cellular bioenergetics, biophysical measurements, proteomics or mitochondrial DNA and lipids analysis.

Preparation of Highly Coupled Rat Heart Mitochondria

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Summary

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