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Protocol for Mosquito Rearing (A. gambiae)
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Biologia
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JoVE Journal Biologia
Protocol for Mosquito Rearing (A. gambiae)

Protocol for Mosquito Rearing (A. gambiae)

DOI:
10.3791/221-v

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13:03 min

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July 04, 2007

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, ,
1Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Capítulos

  • 00:01Introduction
  • 01:08Day 1 – Blood-Feeding Mosquitoes
  • 03:43Day 3 – Mosquitoes Lay Eggs
  • 05:10Day 4 – Eggs are Collected
  • 06:22Day 5-8 – Feed Larvae Every Day
  • 07:36Day 9-10 – Late Stage Larvae
  • 08:02Day 13 – From Larvae to Pupae
  • 08:20Day 14-16 – Adult Mosquitoes Emerge
  • 08:39Day 16 – Collection of Adult Mosquitoes
  • 11:44Day 17-21 – The Cycle Repeats
  • 12:07Conclusion

Summary

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This video illustrates the general techniques used to rear Anopheles gambiae in the laboratory. The methods for caring for laboratory mosquitoes are demonstrated through all stages of the organism's life cycle from larvae to pupae to blood-feeding adults.

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Mosquito RearingA. GambiaeInsectaryTemperatureHumidityDay/night CycleMosquito CagesSterile Sucrose SolutionPaper TowelsBeakerWhatman Filter PaperGlass FeedersHuman BloodSerumWater BathParafilmDistilled WaterPlastic TraysMosquito FoodMosquito NetVacuumCollection Chamber

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