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由紫外线引起的复制中间体的可视化 E。大肠杆菌使用二维的琼脂糖凝胶电泳分析
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Biologia
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JoVE Journal Biologia
Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
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由紫外线引起的复制中间体的可视化 E。大肠杆菌使用二维的琼脂糖凝胶电泳分析

DOI:
10.3791/2220-v

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10:36 min

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December 21, 2010

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, ,
1Department of Biology,Portland State University

Capítulos

  • 00:05Título
  • 00:57Introduction
  • 01:19Cell Growth and UV Irradiation
  • 03:14DNA Isolation
  • 05:502D Gel and Southern Analysis
  • 08:57Representative Results for Visualization of UV-induced Replication Intermediates in E. coli
  • 10:13Conclusion

Summary

Tadução automática

Tadução automática

我们目前可以使用其中的一个两维的琼脂糖凝胶电泳分析，以确定紫外线照射后发生的复制中间体的结构的过程。

Tags

UV-induced Replication IntermediatesE. ColiTwo-dimensional Agarose-gel AnalysisInaccurate ReplicationDNA DamageCellular RearrangementsMutagenesisCancer DevelopmentGene ProductsReplication Encounters DNA LesionsReplicating PlasmidsUV-induced DNA DamageReplication ForksRecARecF PathwayNucleotide Excision Repair

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