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Cryopreserving和恢复人类iPS细胞血清替代馈线中使用完整的基因敲除
JoVE Journal
Biologia
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JoVE Journal Biologia
Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Cryopreserving和恢复人类iPS细胞血清替代馈线中使用完整的基因敲除

DOI:
10.3791/2237-v

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06:47 min

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July 15, 2010

•

,
1GIBCO,Life Technologies

Capítulos

  • 00:04Título
  • 00:44Introduction
  • 01:31Cryopreserving iPSCs
  • 03:48Thawing iPSCs
  • 05:54Representative Results
  • 06:24Conclusion

Summary

Tadução automática

Tadução automática

这一协议描述为cryopreserving淘汰赛SR冻存介质的人类iPS细胞，这些细胞恢复完整的基因敲除SR直属免费（KSR - FF）介质或进纸器为基础的基因敲除的SR介质的详细过程。

Tags

CryopreservingRecoveringHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-free MediumReprogrammingFibroblastsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementEmbryonic Stem Cell GrowthIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Or Human Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated Cells

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