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1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine, Veterans Affairs TVHS,Vanderbilt University School of Medicine
Capítulos
- 00:05Título
- 00:40Introduction
- 01:02Zebrafish Egg Collection and 96-Well Plate Preparatin
- 02:52Small Molecule Library
- 04:23Screening for Effects of Small Molecules by Visual Inspection of Phenotypes
- 05:51Small Molecule Detection with Zebrafish Chemical Screening Assay
- 06:21Conclusion
Le poisson zèbre est apparu comme un puissant<em> In vivo</em> Plate-forme pour les écrans de la drogue basée sur le phénotype et l'analyse chimique génétique. Ici, nous démontrons une méthode simple et pratique pour criblage à grande échelle de petites molécules à partir d'embryons de poisson zèbre.
