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Patch Clamp och tekniker perfusion för att studera jonkanaler Uttryckt i Xenopus Oocyter
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Biologia
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JoVE Journal Biologia
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
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Patch Clamp och tekniker perfusion för att studera jonkanaler Uttryckt i Xenopus Oocyter

DOI:
10.3791/2269-v

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10:19 min

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January 10, 2011

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, , ,
1Department of Energy, Environmental & Chemical Engineering,Washington University in St. Louis, 2Department of Biomedical Engineering,Washington University in St. Louis, 3Department of Biomedical Engineering and Cardiac Bioelectricity and Arrhythmia Center,Washington University in St. Louis

Capítulos

  • 00:05Título
  • 00:45Introduction
  • 01:10Injecting mRNA into Oocytes
  • 01:51Preparing the Perfusion System
  • 04:26Preparing the Ag/AgCl Wire and the Data Acquisition Equipment
  • 05:33Stripping the Vitelline Membrane from the Oocyte
  • 06:14Preparing the Patch Pipettes
  • 07:06Obtaining an Inside-Out Patch
  • 08:08Patch Clamping
  • 08:41Recording of Wild-Type BK Channels on and Oocyte Membrane Patch
  • 09:57Conclusion

Summary

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Tadução automática

Jonströmmen av BK-kanaler spelas in med den teknik patch clamp. BK kanaler uttrycks i<em> Xenopus oocyter</em> Genom att injicera budbärar-RNA. Den intracellulära lösning under inspelningarna patch clamp styrs av en perfusion system.

Tags

Patch ClampPerfusion TechniquesIon ChannelsXenopus OocytesBK ChannelsActivationStructure-function RelationshipNeurotransmitter ReceptorsSmooth Muscle ContractionHair Cell Frequency TuningNeurotransmitter ReleaseMembrane DepolarizationIntracellular Ca2+ And Mg2+Messenger RNA InjectionIncubationInside-out ConfigurationSingle Or Multiple ChannelsRecording CurrentsControlled Voltage

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