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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

DOI:
10.3791/2287-v

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07:19 min

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October 21, 2010

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, , ,
1Department of Biology,University of Texas San Antonio – UTSA, 2South Texas Center for Emerging Infectious Diseases,University of Texas San Antonio – UTSA

Capítulos

  • 00:00Título
  • 01:24Preparation of C. albicans
  • 02:49Setting Up the 96-well Microtiter Plate for the Formation of the Biofilm
  • 03:55Antifungal Susceptibility Testing of Biofilms
  • 06:02Evaluation of Antifungal Reagents
  • 06:43Conclusion

Summary

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We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

Tags

96 Well Microtiter PlateMonitoringFormationAntifungal Susceptibility TestingCandida AlbicansBiofilmsCandidiasisFungal InfectionsMedical DevicesCathetersIn Vitro ModelColorimetric AssayXTTTetrazolium SaltMetabolically Active Fungal Biofilm CellsExperiment DurationAntifungal Susceptibility Testing Standardization

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