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Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
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Biologia
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JoVE Journal Biologia
Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)

Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)

DOI:
10.3791/230-v

•

11:48 min

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July 04, 2007

•

,
1Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Capítulos

  • 00:01Introduction
  • 00:24Preparation of Mosquitoes and Work Area
  • 01:47injecting Mosquitoes with dsRNA
  • 08:53Treatment of Mosquitoes after Injection
  • 11:11Conclusion
  • 11:37End Credits

Summary

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Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.

Tags

RNAi AssaysAdult MosquitoesA. GambiaeReverse GeneticsVector PathogensResistance GenesDimopoulos LabDsRNA InjectionAnopheles GambiaeMalaria ParasiteInjection Setup

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