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Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

DOI:
10.3791/2308-v

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09:10 min

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October 18, 2010

•

,
1Center for Meat Safety and Quality, Department of Animal Sciences,Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition,Iowa State University

Capítulos

  • 00:00Título
  • 01:32Introduction
  • 02:07Surface Sampling with Sterile Adhesive Tape
  • 03:33Solid-phase Enrichment
  • 04:54Fixation and Dehydration
  • 05:35Hybridization
  • 06:46Detection
  • 07:43FISH and FACS Analyses on Tomatoes
  • 08:32Conclusion

Summary

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This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).

Tags

Adhesive-tape-based SamplingFluorescence In Situ HybridizationRapid DetectionSalmonellaFresh ProduceCulture-independent DetectionCell-charged TapesSolid-phase EnrichmentLiquid Surface MinicultureDNA Probe CocktailHybridizationFluorescence Microscopy

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