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Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
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Neurociência
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JoVE Journal Neurociência
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth

Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth

DOI:
10.3791/2309-v

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05:42 min

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March 29, 2011

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1Interdisciplinary Center for Neurosciences, Institute of Anatomy and Cell Biology,University of Heidelberg

Capítulos

  • 00:05Título
  • 01:20Preparation for Slicing and Culturing
  • 02:08Embryo Embedding
  • 03:18Slicing Procedure
  • 04:23Imaging Spinal Nerve Outgrowth
  • 04:54Representative Results of Imaging Spinal Nerve Outgrowth
  • 05:21Conclusion

Summary

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We present a method to prepare organotypic slices of mid-gestation mouse embryos for the cultivation and time-lapse imaging of peripheral nerve outgrowth.

Tags

Organotypic Slice CultureGFP-expressing Mouse EmbryosReal-time ImagingPeripheral Nerve OutgrowthEx Vivo CultivationTransgenic Mouse LineTauGFPGreen Fluorescent ProteinNeuronsCentral Nervous SystemPeripheral Nervous SystemForelimb InnervationPharmacological TechniquesGenetic TechniquesVibratomeSlice PreparationViabilityAge-specific DevelopmentSpinal NervesSpinal CordDorsal Root GangliaEmbryonic Day (E) E10 To E12 Slices

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