/
/
Real-time Imaging von Leukotrien B 4 Vermittelte Zellmigration und BLT1 Wechselwirkungen mit β-Arrestin
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:13Introduction
- 01:48Preparation of Bone Marrow Derived Dendritic Cells (BMDC)
- 02:42Preparation of Lipid Loaded Micropipette
- 04:20Live-cell Imaging of Cell Migration in Response to Ligand Application
- 05:25Representative Results for Migration of BMDCs
- 06:03Live Cell Imaging of Transfected RBL-2H3 Cells
- 09:49Image Analysis
- 10:51Representative Results
- 13:03Conclusion
Dieses Papier beschreibt die Methodik auf die chemotaktische Reaktion von Leukozyten an spezifische Liganden zu bestimmen und zu identifizieren Wechselwirkungen zwischen den Rezeptoren auf der Zelloberfläche und zytosolischen Proteinen unter Verwendung von Live Cell Imaging Verfahren.
Tags
Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway
