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Isolation and In vitro Activation of Caenorhabditis elegans Sperm
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Biologia
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JoVE Journal Biologia
Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

DOI:
10.3791/2336-v

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05:46 min

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January 31, 2011

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, , ,
1Waksman Institute of Microbiology,Rutgers University

Capítulos

  • 00:05Título
  • 00:21Subscription
  • 00:40Introduction
  • 01:29Enrichment of Male Population, Identification, and Isolation of Males
  • 02:29Dissection of Males and in vitro Activation
  • 04:25Visualization of Spermtids and Spermatozoa
  • 04:54Images of C. elegans Spermatid Isolation and Activation
  • 05:21Conclusion

Summary

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Tadução automática

A protocol for isolating and activating spermatids from male C. elegans is described here. Cutting the posterior end of male releases spermatids. The spermatids can be activated by addition of protease.

Tags

Caenorhabditis ElegansIsolationIn Vitro ActivationSpermMalesHermaphroditesGametogenesisSpermathecaOocytesX Chromosome Non-disjunctionHim PhenotypeTail MorphologyMating StructuresDissectionStereo MicroscopeSpermatids

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