Journal
/
Biologia
/
使用胚体冰冻切片分析的多能干细胞
JoVE Journal
Biologia
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
Please note that all translations are automatically generated. Click here for the English version.

使用胚体冰冻切片分析的多能干细胞

DOI:
10.3791/2344-v

•

06:54 min

•

December 08, 2010

•

, , , ,
1Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro (UFRJ), Brazil

Capítulos

  • 00:02Título
  • 00:07Introduction
  • 00:37Materials and Equipment
  • 01:09Preparing Embryoid Body Cryo-Blocks
  • 05:14Cutting
  • 06:37Conclusion

Summary

Tadução automática

Tadução automática

多能干细胞悬浮液中生长分化成胚状体（EBS）。在这里，我们展示了如何获得高品质的EB冰冻切片，用于研究胚胎发育的细胞和分子方面，同时保留其作为集合体的组织。

Tags

Pluripotent Stem CellsCryosectionsEmbryoid BodiesES CellsDifferentiationAggregatesEctodermMesodermEndodermImmunofluorescenceIn Situ HybridizationTarget ProteinsMRNACryosection TechniqueAnalytical ProceduresHistology Staining AnalysisHematoxilin And EosinDAPIOct4NestinEmbryogenesisTri-dimensional Spherical Structure

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/2344?article_type=v

Shrinky丁克挂滴：一个简单的方法，形式和文化胚体
check_url/pt/2344?article_type=v

由Stemgent人类TF慢病毒重新编程人类成纤维细胞生成诱导多能干细胞
check_url/pt/2344?article_type=v

人多能干细胞的免疫组织化学分析，使用自制Cytospin器械
check_url/pt/2344?article_type=v

人类诱导多能干细胞的转染和Nucleofecting
check_url/pt/2344?article_type=v

人类体细胞重编程为诱导多能干细胞（iPS细胞）用绿色荧光蛋白逆转录病毒载体
check_url/pt/2344?article_type=v

诱导多能干细胞来源于小鼠的生成
check_url/pt/2344?article_type=v

代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体
check_url/pt/2344?article_type=v

诱导上皮和分析间质转化
check_url/pt/2344?article_type=v

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪
check_url/pt/2344?article_type=v

人类多能干细胞的高效分化为心肌细胞，并鉴定流式细胞仪

Read Article