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在人T淋巴细胞的全细胞钙释放激活的钙记录（CRAC）的电流

JoVE Journal
Biologia

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JoVE Journal Biologia

Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes

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在人T淋巴细胞的全细胞钙释放激活的钙记录（CRAC）的电流

DOI:

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15:29 min

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December 21, 2010

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Pratima Thakur, Alla F. Fomina

¹Department of Physiology and Membrance Biology,University of California, Davis

Capítulos

00:05Título
02:04Experimental Preparation
04:15Mounting Cells on the Microscope Stage
05:25CRAC Channel Current Recording
11:43Data Analysis
12:20Representative CRAC Channels Recorded in a Resting Human T Lymphocyte
14:39Conclusion

Summary

Tadução automática

钙离子释放活化钙全细胞膜片钳记录（CRAC）外周血单核细胞衍生的人类T淋巴细胞中的电流，我们提供了一步一步的协议。

Tags

Whole-cell Recording Calcium Release-activated Calcium Currents CRAC Channels T Lymphocytes Intracellular Ca2+ Store Plasmalemmal Ca2+ Channels T Cell Proliferation Gene Expression Severe Combined Immunodeficiency Autoimmune Diseases Functional Channel Properties Regulation Jurkat T Cells Human Leukemia T Cell Line Normal Human T Cells Blood-derived T Lymphocytes Amplitude Ca2+ Currents Na+ Currents

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