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Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

DOI:
10.3791/2363-v

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07:12 min

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November 24, 2010

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, , , ,
1Microbiology, Immunology, and Pathology,Colorado State University

Capítulos

  • 00:05Título
  • 01:38per os Infection of Mosquitoes
  • 03:28Monitoring Infection of Mosquitoes
  • 04:18Transmission Assay (Forced salivation to demonstrate virus transmission)
  • 05:30Alphavirus Infection of Mosquito Cell Culture and the Adult Female Mosquito
  • 06:48Conclusion

Summary

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Methods for using alphavirus transducing systems to express fluorescent reporters in vitro and in adult mosquitoes are described. This technique may be adapted to express any protein of interest in lieu of or in addition to a reporter.

Tags

Alphavirus Transducing SystemVisualizing InfectionMosquito VectorsGene ExpressionMosquito-borne RNA VirusesAlphavirus GenusCDNA ClonesSingle-stranded RNA GenomesStructural ProteinsGene InsertionViral Subgenomic RNA Initiation SitePromoterStable Protein ExpressionRNAiGene Silencing

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