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Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
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Biologia
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JoVE Journal Biologia
Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

DOI:
10.3791/237-v

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13:26 min

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July 19, 2007

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,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capítulos

  • 00:00Título
  • 00:05Introduction
  • 01:00Tools and Reagents
  • 02:28Preparing the Emulsion
  • 06:48Immunizing Rats with Emulsion
  • 09:37Challenging Rats with Relevant and Irrelevant Antigen
  • 11:04Measuring Inflammation
  • 12:40Conclusion

Summary

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Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes. Here we demonstrate how to induce active DTH in Lewis rats and monitor the inflammatory response.

Tags

InductionMonitoringActive Delayed Type Hypersensitivity (DTH)RatsInflammatory ReactionCCR7- Effector Memory T LymphocytesAntigenImmune SystemPrimedRednessSwellingImmunosuppressantsCutaneous DTHAdoptive TransferActive ImmunizationOvalbuminTuberculinTetanus ToxoidKeyhole Limpet Hemocyanin (KLH)Water-in-oil EmulsionComplete Freund’s AdjuvantSubcutaneouslyPrime Immune SystemMemory T CellsChallenge IntradermallyVoltarIrrelevant AntigenVisible ReactionSeverity

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