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在体外的tRNA与阿米巴 DNA和tRNA甲基Dnmt2（Ehmeth）酶甲基化含量
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
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在体外的tRNA与阿米巴 DNA和tRNA甲基Dnmt2（Ehmeth）酶甲基化含量

DOI:
10.3791/2390-v

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12:36 min

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October 19, 2010

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, , ,
1Faculty of Medicine, Rappaport Institute,Technion – Israel Institute of Technology, 2The Pharmacy and Biochemistry Institute,Johannes Gutenberg University

Capítulos

  • 00:00Título
  • 01:43tRNA Preparation
  • 05:28Expression and Purification of the Recombinant E. histolytica Dnmt2 (Ehmeth) Protein in E. coli BL21
  • 08:06 in vitro tRNA Methylation Assay
  • 10:30Representative Results
  • 12:02Conclusion

Summary

Tadução automática

Tadução automática

这个协议描述为合成tRNA的衬底准备<em>阿米巴</em>的tRNA / DNA的甲基转移酶2（Dnmt2）的同源性Ehmeth和甲基转移酶活性的措施。这种实验方法可用于调查其他Dnmt2蛋白的活性。

Tags

In Vitro TRNA Methylation AssayEntamoeba HistolyticaDNA Methyltransferase Dnmt2Ehmeth EnzymeProtozoan ParasitesAmebiasisInfectious AgentsClinical Manifestations5-methylcytosine (5mC)Single DNA MethyltransferaseControl Of Repetitive ElementsDual DNA/tRNAAsp Methyltransferase ActivityFunctional SignificanceTRNA Methyltransferase ActivityDnmt2 Proteins

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