Journal
/
Imunologia e Infecção
/
一个负选择标记的发展阿米巴</em
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Development of a Negative Selectable Marker for Entamoeba histolytica
Please note that all translations are automatically generated. Click here for the English version.

一个负选择标记的发展阿米巴</em

DOI:
10.3791/2410-v

•

06:05 min

•

December 12, 2010

•

, , ,
1Division of Infectious Disease and International Health,University of Virginia Health System

Capítulos

  • 00:05Título
  • 00:58Introduction
  • 01:37FCU Negative Selection System in Entamoeba histolytica
  • 03:23Determination of Selection Efficiency
  • 04:38The FCU1/5-FC System: A Powerful Negative Selection Tool for E. histolytica Trophozoites
  • 05:43Conclusion

Summary

Tadução automática

Tadução automática

我们的报告负选择系统的发展<em> E。阿米巴</em>基于嵌合蛋白的转基因表达（FCU1）和前体药物5 - 氟胞嘧啶的选择。 FCU1蛋白是一种融合酵母胞嘧啶脱氨酶和尿嘧啶phosphoribosyltransferase。 FCU1表达导致增加<em> E。阿米巴</em>对5 - 氟胞嘧啶的敏感性。

Tags

Negative Selectable MarkerEntamoeba HistolyticaAmebiasisGene ExpressionTransfectionPlasmidsReverse GeneticsHomologous RecombinationEpisomal Plasmid DNABackground Of Exogenous DNAGenomic Integration EventNegative Selection SystemYeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil5-fluorouridine-5′-monophosphate

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/2410?article_type=v

在体外的tRNA与阿米巴 DNA和tRNA甲基Dnmt2（Ehmeth）酶甲基化含量
check_url/pt/2410?article_type=v

考试流式细胞仪检测胸腺阳性和阴性选择
check_url/pt/2410?article_type=v

使用点击化学测量的影响，病毒感染宿主细胞RNA合成
check_url/pt/2410?article_type=v

革兰从血培养液使用MALDI-TOF质谱阴性细菌快速鉴定
check_url/pt/2410?article_type=v

上皮损伤制作分析<em>阿米巴</em>感染
check_url/pt/2410?article_type=v

在乳腺癌检测人类白细胞抗原生物标志物的利用无标记生物传感技术
check_url/pt/2410?article_type=v

可视化痰生物膜发展影响使用腔室盖玻片型号
check_url/pt/2410?article_type=v

乙型肝炎病毒记者系统的开发监控复制周期的早期阶段
check_url/pt/2410?article_type=v

利用胶囊对生物达标中病毒样品的阴性染色
check_url/pt/2410?article_type=v

Monospecific 和混合 DFS70 模式在 HEp-2 ELITE/DFS70 击倒底物筛选中的同时鉴别

Read Article