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酶标菜生物膜形成实验
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Microtiter Dish Biofilm Formation Assay
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酶标菜生物膜形成实验

DOI:
10.3791/2437-v

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03:57 min

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January 30, 2011

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1Microbiology and Immunology,Dartmouth Medical School

Capítulos

  • 00:05Título
  • 00:54Growing Biofilms
  • 01:28Staining Biofilms
  • 02:44Quantifying the Biofilms
  • 03:23Quantification of Biofilms Formed by P. fluorescens Over Time
  • 03:37Conclusion

Summary

Tadução automática

Tadução automática

实验描述了一个迅速的手段来衡量早期形成生物膜的细菌和真菌。此方法使用一个微孔板，微生物形成生物膜的基质和生物膜是可视化，使用龙胆紫应变。检测提供了一个定性或定量检测早期生物膜的形成。

Tags

Microtiter DishBiofilm Formation AssayMicrobesSurfacesMedicalIndustrialNatural SettingsExtracellular MatrixArchitectureMacrocoloniesFluid-filled ChannelsResistance To Antimicrobial AgentsAntibioticsEarly StagesBacterial BiofilmsFungal Biofilm FormationStatic Batch-growth ConditionsMature BiofilmsFlow Cell SystemsInitiation Of Biofilm FormationFlagellaPiliAdhesinsCyclic-di-GMP Binding And MetabolismExtracellular Polysaccharide Production

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