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酶谱法检测功能的基质金属蛋白酶
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Detection of Functional Matrix Metalloproteinases by Zymography
DOI:

09:30 min

November 08, 2010

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Capítulos

  • 00:00Título
  • 00:54Introduction
  • 01:23Zymography Gel Electrophoresis
  • 03:43Renaturing and Developing the Gel
  • 06:07Data Analysis
  • 08:03Representative Results for the Detection of MMP-2 using Zymography
  • 09:00Conclusion

Summary

Tadução automática

本协议描述了一个用于检测培养上清或体液基质金属蛋白酶的活动为基础的检测。

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