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Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
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JoVE Journal Neurociência
Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

DOI:
10.3791/2457-v

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11:54 min

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January 11, 2011

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, , , ,
1Department of Anatomical Sciences,Shiraz University of Medical Sciences, Shiraz, Iran , 2Department of Neurosurgery,The University of Florida

Capítulos

  • 00:01Título
  • 00:29Introduction
  • 00:57Animal Dissection
  • 02:40E14 Brain Dissection
  • 04:42Tissue Dissociation
  • 08:00Passaging Neurospheres
  • 10:10Representative Results
  • 11:31Conclusion

Summary

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This video protocol demonstrates the application of the neurosphere assay for the isolation and expansion of neural stem cells from the ganglionic eminences of embryonic day 14-mouse brain.

Tags

Embryonic Mouse Neural Stem Cell CultureNeurosphere AssayStem CellsUndifferentiated CellsCell GenesisAging ProcessInjury And DiseaseNeural Stem Cells (NSCs)Serum-free Culture SystemIsolation And Expansion Of NSCsEmbryonic CNSIn Vitro Expanded NSCsCNS Cell TypesReliable And Consistent ResultsE14 Mouse EmbryosBrain MicrodissectionGanglionic EminencesSingle Cell SuspensionNSA CulturePrimary Neurospheres

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