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JoVE Journal Neuroscience
An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
 
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An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Article DOI: 10.3791/2486-v • 10:41 min • December 11th, 2010
December 11th, 2010 •
1, 1, 1
1Department of Molecular Biomedical Sciences, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University

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This protocol describes an organotypic slice assay optimized for the postnatal brain and high-resolution time-lapse imaging of neuroblast migration in the rostral migratory stream.

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Organotypic Slice Assay High-resolution Time-lapse Imaging Neuronal Migration Postnatal Brain Neurogenesis Progenitor Cells Subependymal Zone (SEZ) Rostral Migratory Stream (RMS) Olfactory Bulbs (OB) Cellular Mechanisms Molecular Mechanisms Physiological Mechanisms Extracellular Conditions Migrating Neuroblasts Uniform Movement Oriented Movement Speedy Movement Cell Autonomous Regulation Non-autonomous Regulation Cross-transplantation Approaches
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