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Imunologia e Infecção
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Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

Expansion, Purification, and Functional Assessment of Human Peripheral Blood NK Cells

DOI:
10.3791/2540-v

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10:44 min

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February 02, 2011

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, , ,
1Division of Pediatrics,MD Anderson Cancer Center – University of Texas

Capítulos

  • 00:05Título
  • 01:13Introduction
  • 01:40Isolation of Cell Fractions from Buffy Coat
  • 03:34NK Cell Expansion
  • 06:34NK Cell Cytotoxicity Assay
  • 08:33Representative Results
  • 09:55Conclusion

Summary

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Here we describe a method to efficiently expand and purify large numbers of human NK cells and assess their function.

Tags

ExpansionPurificationFunctional AssessmentHumanPeripheral BloodNK CellsNatural Killer CellsImmune SurveillanceInfectious MicroorganismsTumorsLimited AvailabilityIn VitroNK Cell ImmunotherapyMethodEfficiently ExpandVast QuantitiesFunctional NK CellsEx VivoK562 Cells Expressing Membrane-bound IL21Artificial Antigen-presenting Cell (aAPC)Cell ProductSteady-state LeukapheresisDonorDepletion Of T CellsPositive Selection Of NK CellsIL-2 Overnight ActivationSmall Numbers Of NK Cells Delivered In Clinical TrialsInability To Propagate NK Cells In VitroGenerating Sufficient Cell Numbers

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