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Alta MicroRNA profiling Throughput: Multiplex Ottimizzato qRT-PCR a scala nanoliter sul Fluidigm dinamica IFCS ArrayTM
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1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California San Francisco , 2Center for Reproductive Sciences,University of California San Francisco , 3Department of Urology,University of California San Francisco , 4Department of Cell and Tissue Biology,University of California San Francisco , 5Fluidigm Corporation,Fluidigm Corporation , 6Department of Obstetrics and Gynecology,Hadassah-Hebrew University Medical Center, 7UCSF – Helen Diller Family Comprehensive Cancer Center,University of California San Francisco
Capítulos
- 00:05Título
- 00:55RNA Concentration
- 02:03Gel Purification of pre-PCR Product
- 03:46Preparing the 96.96 Dynamic Array IFC, the Samples, and the 13x Assays
- 05:25qRT-PCR Analysis of miRNA Expression in Mouse ESC and Human Serum
- 06:10qRT-PCR Analysis
- 07:04Conclusion
Qui descrivere un ottimizzato trascrittasi inversa multiplex PCR quantitativa (QRT-PCR) protocollo in combinazione con una piattaforma microfluidica come un efficace costi e tempi high-throughput screening per microRNA (miRNA) livelli di espressione, soprattutto quando si lavora con quantità limitate di campione.
Tags
High Throughput MicroRNA ProfilingMultiplex QRT-PCRFluidigm Dynamic ArrayTM IFCsMiRNAsQuantificationMicroRNA LevelsWild-typeSmall RNA Deficient Mouse Embryonic Stem Cells (mESC)AccuracyRobustnessOptimized MethodPurifying PrimersSingleplex Real-time DetectionMicrofluidic ChipNanoliter VolumesReagent CostsHigh Throughput MiRNA Expression Profiling
