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Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny

Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny

DOI:

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08:36 min

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June 07, 2011

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Maureen R. Lynch, Judith C. Gasson, Helicia Paz

¹Department of Medicine, Hematology-Oncology,University of California, Los Angeles, ²Department of Biological Chemistry,University of California, Los Angeles

Capítulos

00:05Título
01:31Preparing mStrawberry OP9 Cells
02:32Setting up ES/mStrawberry OP9 Co-culture (Day 0)
03:55Maintaining ES/mStrawberry OP9 Co-culture
06:16Evaluating ES-derived Progeny from the Co-culture System

Summary

Tadução automática

mStrawberry OP9 cells allow for complete evaluation of all ES-derived progeny from co-culture.

Tags

ES/OP9 Co-culture Protocol Hematopoietic Progeny In Vitro Differentiation Early Hematopoietic Development Flk-1+ Hemangioblasts Terminally Differentiated Lineages OP9 Stromal Cells M-CSF Embryonic Lethalities Macrophage Overproduction Replating Steps Evaluation Of ES-derived Progeny

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