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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
JoVE Journal
Biologia
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JoVE Journal Biologia
Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

DOI:
10.3791/2560-v

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04:54 min

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April 19, 2011

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1Department of Biology and Biotechnology,Worcester Polytechnic Institute- WPI

Capítulos

  • 00:05Título
  • 00:55Making Protoplasts
  • 02:14Transformation of DNA into Protoplasts
  • 04:30Conclusion

Summary

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Tadução automática

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation1.

Tags

EfficientPolyethylene Glycol (PEG)TransformationMossPhyscomitrella PatensProtoplastsModel SystemMitotic Homologous RecombinationGene TargetingDNA DeliveryParticle BombardmentPEG-mediated DNA UptakeTransformation EfficiencyGene GunsSterile HoodTransient TransformantsReproducibility

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