Journal
/
Neurociência
/
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

DOI:
10.3791/2567-v

•

09:36 min

•

March 23, 2011

•

, ,
1Institute of Neurobiology,Ulm University, 2School of Computing Science & Institute of Neuroscience,Newcastle University

Capítulos

  • 00:05Título
  • 02:11Preparing the Voltage-Sensitive Dye Solution
  • 03:18Preparing the Stomatogastric Ganglion and the Extracellular Recording
  • 03:58Dye Loading by Intracellular Injection
  • 06:35Imaging of Dyed Neurons
  • 08:03Results: Optical and Electrical Recordings from STG Neurons
  • 08:57Conclusion

Summary

Tadução automática

Tadução automática

Here we present the methodology for fast and high resolution fluorescent voltage-sensitive dye imaging of detailed activity of neurons in the crab stomatogastric ganglion.

Tags

Optical ImagingNeuronsCrab Stomatogastric GanglionVoltage-sensitive DyesMethodologyNeuronal NetworksSimultaneous ActivityIntegral FunctionalityNetwork OrganizationPattern Generating NeuronsFluorescent Voltage-sensitive DyeDi-8-ANEPPQMiCAM02 High Speed And High Resolution CCD Camera Imaging SystemBVAna Imaging SoftwareElectrophysiology TechniquesCentral Pattern Generator Neural Networks

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/2567?article_type=v

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
check_url/pt/2567?article_type=v

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
check_url/pt/2567?article_type=v

Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells
check_url/pt/2567?article_type=v

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes
check_url/pt/2567?article_type=v

3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii
check_url/pt/2567?article_type=v

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
check_url/pt/2567?article_type=v

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
check_url/pt/2567?article_type=v

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice
check_url/pt/2567?article_type=v

Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye
check_url/pt/2567?article_type=v

Isolation and Culture of Chick Ciliary Ganglion Neurons

Read Article