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改性膜联蛋白V /碘化丙啶检测细胞凋亡细胞死亡的准确评估
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
DOI:

07:34 min

April 24, 2011

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Capítulos

  • 00:05Título
  • 01:34Cell Preparation
  • 02:14Application of Annexin V/PI Stain
  • 04:26RNase-treated Cells and Cell Lines Exhibit Fewer False Positive Staining Results
  • 06:56Conclusion

Summary

Tadução automática

为评估细胞死亡的一种准确的方法是描述。该协议改进传统的膜联蛋白V /碘化丙碘化物(PI)的协议,这显示多达40%的假阳性的细胞株和原代细胞的动物模型的广泛事件。

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