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人类神经干细胞计数
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Biologia
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JoVE Journal Biologia
Counting Human Neural Stem Cells
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人类神经干细胞计数

DOI:
10.3791/262-v

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06:37 min

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August 22, 2007

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,
1Department of Pathology,University of California, Irvine (UCI)

Capítulos

  • 00:00Título
  • 00:12Passaging Human Embryonic Neural Stem Cells
  • 00:30Preparing the Cell Suspension for Counting
  • 03:54Counting Cells in the Hemocytometer

Summary

Tadução automática

Tadução automática

知识是需要许多组织培养操作活菌的确切人数。本协议描述了如何区分活细胞和死细胞和使用hemacytometer量化细胞。虽然它描述了人类神经干/前体细胞（hNSPCs）计数，它可用于其他类型的细胞。

Tags

CountingHuman Neural Stem CellsViable CellsCell SuspensionImmunocytochemistryCell TransfectionsHemacytometerLive CellsDead CellsCell ConcentrationTotal Cell NumberTrypan BlueQuadrantsDilution Factor

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