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Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
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Biologia
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JoVE Journal Biologia
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

DOI:
10.3791/2620-v

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09:53 min

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May 20, 2011

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,
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center,National Heart, Lung, and Blood Institute, National Institutes of Health

Capítulos

  • 00:05Título
  • 01:42Collecting Embryonic Limb Skins
  • 03:53Whole-mount Immunohistochemical Staining of Limb Skins
  • 07:14Mounting the Limb Skins on a Slide
  • 08:24Results of Whole-mount Confocal Immunofluorescence Microscopy
  • 09:29Conclusion

Summary

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We introduce a whole-mount immunohistochemistry and laser scanning confocal microscopy with multiple labelling for analyzing intricate vascular network formation in mouse embryonic limb skin.

Tags

Whole-mount Immunohistochemical AnalysisEmbryonic Limb Skin VasculatureVascular Branching MorphogenesisVascular DevelopmentLimb Skin Vasculature ModelWhole-mount Confocal MicroscopyFluorescent MarkersGenetic StudiesMolecular MechanismsPeripheral NervesSpatial TemplateDifferentiation And Patterning Of ArteriesStaining Intact Blood VesselsVascular Specific AntibodiesFluorescent Secondary AntibodiesVascularized Embryonic Organs

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