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Associated Chromosome Trap for Identifying Long-range DNA Interactions
JoVE Journal
Biologia
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JoVE Journal Biologia
Associated Chromosome Trap for Identifying Long-range DNA Interactions

Associated Chromosome Trap for Identifying Long-range DNA Interactions

DOI:
10.3791/2621-v

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14:49 min

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April 23, 2011

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,
1Medical Service, VA Palo Alto Health Care System ,Stanford University School of Medicine

Capítulos

  • 00:05Título
  • 01:30Formaldehyde Fixation of Long-Range Chromatin Interactions and Nuclei Isolation
  • 03:36Digestion, Ligation and Purification of Interaction DNA Segments
  • 06:10Digestion with Msp I and Ligation with Oligonucleotide Linkers
  • 07:50PCR Amplification and Sequence Analysis
  • 11:06Act Assay Representative Results
  • 14:23Conclusion

Summary

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Tadução automática

The associated chromosome trap (ACT) assay is a novel unbiased method for identifying long-range DNA interactions. The characterization of long range DNA interactions will allow us to determine the relationship of nuclear architecture to gene expression in both normal physiology and in diseased states.

Tags

Associated Chromosome TrapLong-range DNA InteractionsChromatin StructureChromosome TerritoriesGene ExpressionTranscriptional FactoriesCo-localizationACT AssayChromosome Conformation Capture TechniqueTranscriptional RegulationNuclear ArchitectureGene Expression In Disease States

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