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Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels
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Biologia
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JoVE Journal Biologia
Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

DOI:
10.3791/264-v

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22:45 min

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August 22, 2007

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,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Capítulos

  • 00:00Título
  • 00:11Introduction
  • 00:59Loading and Running an the SDS-PAGE Gel
  • 08:12Transferring Proteins to Nitrocellulose Membrane
  • 15:34Immunoblotting
  • 20:22Analyzing Western Blot
  • 22:18Conclusion

Summary

Tadução automática

Tadução automática

This technical article describes a standard western-blotting procedure using the commercially available NuPAGE electrophoresis Mini-Gel system from Invitrogen.

Tags

Western BlottingInvitrogen NuPage Novex Bis Tris MiniGelsImmunoblottingProtein ExpressionProtein AnalysisCo-immunoprecipitationPrimary AntibodyProtein SeparationMolecular WeightSDS-PAGEMembrane TransferChemiluminescencePre-cast GelsUser-friendly EquipmentNuPAGE Electrophoresis SystemLaemmli TechniqueShelf LifeMolecular Weight RangeAcrylamide PercentageRunning Buffer

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