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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
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Biologia
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JoVE Journal Biologia
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

DOI:
10.3791/2645-v

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09:38 min

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May 31, 2011

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, , ,
1Department of Cell and Molecular Biology,Northwestern University

Capítulos

  • 00:05Título
  • 01:27Preparation of DNA and MDCK Cells
  • 02:04Microinjection Procedure and Post-injection Incubations
  • 06:07Surface Staining for Immunofluorescence Analysis
  • 08:21VSVGts045-GFP Control in MDCK Cells
  • 09:05Conclusion

Summary

Tadução automática

Tadução automática

This article details the procedures involved in overexpression and analysis of small GTPases in polarized epithelial cells using microinjection technique.

Tags

Small GTPasesMicroinjectionPlasmidsPolarized Epithelial CellsApical DomainBasolateral DomainTight JunctionsMDCK CellsCell PolarityRas SuperfamilyRalACdc42Rab8Rab10Rab13GDP-bound StateGTP-bound StateNucleotide Binding RegionsDominant NegativeDominant Active AllelesOverexpression

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