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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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JoVE Journal Bioengenharia
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

DOI:
10.3791/2646-v

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11:22 min

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June 14, 2011

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1Department of Biomedical Engineering,Yale University, 2Department of Anesthesiology,Yale University School of Medicine

Capítulos

  • 00:05Título
  • 01:46Preparation of Polyglycolic Acid (PGA) Scaffold
  • 02:55Assembly of Bioreactor
  • 04:33Bioreactor Setup and Seeding Cells
  • 07:55Turning on the Pump, and First Feeding
  • 09:17Staining of Engineered Smooth Muscle Vessels
  • 10:11Conclusion

Summary

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Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.

Tags

Biological-based Vascular GraftsPulsatile BioreactorArterial Bypass RegenerationVessel RegenerationBioreactor Culture SystemsSmooth Muscle Cells (SMCs)Polyglycolic Acid (PGA) MeshSilicone TubingPulsatile StimulationChemo-mechanical EnvironmentNative VesselsEndothelial Cells (EC)Animal Implantation Models

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