Journal
/
Neurociência
/
幼虫和成人测绘和增强子陷阱Flippase表达中的应用果蝇中枢神经系统
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Mapping and Application of Enhancer-trap Flippase Expression in Larval and Adult Drosophila CNS
Please note that all translations are automatically generated. Click here for the English version.

幼虫和成人测绘和增强子陷阱Flippase表达中的应用果蝇中枢神经系统

DOI:
10.3791/2649-v

•

09:45 min

•

June 03, 2011

•

, , , , , , , ,
1Department of Zoology,University of Oklahoma – Norman, 2Department of Biology,Brandeis University

Capítulos

  • 00:05Título
  • 01:45Mapping Transgenic Flippase CNS Expression Patterns in Larva
  • 03:37Mapping Transgenic Flippase CNS Expression Patterns in Adults
  • 05:57Determining the Cell Types in which a Transgenic Flippase Expresses
  • 06:57Utilizing FLP Expression in Photoreceptors from ET-FLP-x2 Lines
  • 08:12Conclusion

Summary

Tadução automática

Tadução automática

我们描述一个Flippase引起的交互联系Gal80/Gal4镇压（FINGR）方法，允许组织特异性FLP确定Gal80表达模式。无论GAL4和FLP重叠，Gal4的表达是开启（Gal80翻转出）或关闭（Gal80翻转）。 FINGR方法是多才多艺的克隆分析和神经回路映射。

Tags

Gal4/UAS Binary MethodGene ManipulationNeural Circuitry ManipulationFINGR MethodGal4 ExpressionGal80 Converting ToolsEnhancer-trap FLPUAS-effectorUAS-reporterMorphological AnalysisTubP>Gal80> (‘flip Out’)TubP>stop>Gal80 (‘flip In’)

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/2649?article_type=v

3幼虫节段性神经的可视化 RD龄果蝇幼虫的筹备工作
check_url/pt/2649?article_type=v

针对嗅球神经元采用组合在体内电穿孔和GAL4基于增强陷阱斑马鱼线
check_url/pt/2649?article_type=v

幼虫，蛹和成人的解剖和免疫组化<em>果蝇</em>视网膜
check_url/pt/2649?article_type=v

果蝇嗅觉成人学习的冲击
check_url/pt/2649?article_type=v

谷氨酸和GABA快速微离子导入：一个有用​​的工具，调查突触整合
check_url/pt/2649?article_type=v

听觉皮层在成年猫用高场磁共振成像功能成像
check_url/pt/2649?article_type=v

在幼虫馈电电路的功能分析果蝇</i
check_url/pt/2649?article_type=v

的实时成像<em>果蝇</em>幼虫神经母细胞
check_url/pt/2649?article_type=v

收购高品质的数字视频<em>果蝇</em>从横向角度看幼虫及成虫行为
check_url/pt/2649?article_type=v

用线性琼脂糖渠道研究<em>果蝇</em>幼虫爬行行为

Read Article