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1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine,Veterans Administration TVHS
Capítulos
- 00:05Título
- 01:30Embryonic Body (EB) Formation Using 96-round Bottom Well Microtiter Plates
- 03:24Identification of Critical Time Windows of Key Signaling Pathway(s) for Cardiogenesis
- 05:04Verification of Time Windows of Signaling for Cardiogenesis in EBs Made from Hanging Dropets
- 07:06Determination of Critical Time Points for ES Cardiogenesis
- 07:45Conclusion
We describe the use of a mouse ES cell based assay to identify critical time windows for Wnt/β-catenin and BMP signal activation during cardiogenic induction. The method provides a standardized platform that reliably quantifies cardiogenic efficiency, and it is applicable to the study of other cell lineages.
Tags
Modified Mouse Embryonic Stem Cell AssayQuantifying Cardiogenic Induction EfficiencyPluripotent Stem CellsKey Signaling PathwaysTemporal RegulationSpatial RegulationDifferentiation EfficiencyWnt/β-catenin SignalingBMP SignalingContracting Embryonic Bodies96-well Plate FormatCardiogenic EfficiencyModulator TreatmentsTime CourseCell LineagesHigh Throughput AssayAutomated Assay
