This content is Free Access.
1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine,Veterans Administration TVHS
Capítulos
- 00:05Título
- 01:30Embryonic Body (EB) Formation Using 96-round Bottom Well Microtiter Plates
- 03:24Identification of Critical Time Windows of Key Signaling Pathway(s) for Cardiogenesis
- 05:04Verification of Time Windows of Signaling for Cardiogenesis in EBs Made from Hanging Dropets
- 07:06Determination of Critical Time Points for ES Cardiogenesis
- 07:45Conclusion
Wir beschreiben die Verwendung einer Maus ES-Zell-basierte Assays, um kritische Zeitfenster für Wnt / β-Catenin und BMP-Signal Aktivierung während kardiogenen Induktion zu identifizieren. Die Methode bietet eine standardisierte Plattform, die zuverlässig quantifiziert kardiogenen Effizienz, und es ist für das Studium der anderen Zelllinien.
Tags
Modified Mouse Embryonic Stem Cell AssayQuantifying Cardiogenic Induction EfficiencyPluripotent Stem CellsKey Signaling PathwaysTemporal RegulationSpatial RegulationDifferentiation EfficiencyWnt/β-catenin SignalingBMP SignalingContracting Embryonic Bodies96-well Plate FormatCardiogenic EfficiencyModulator TreatmentsTime CourseCell LineagesHigh Throughput AssayAutomated Assay
