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荧光光谱法测定膜蛋白质折叠的热力学

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

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荧光光谱法测定膜蛋白质折叠的热力学

DOI:

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10:09 min

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April 28, 2011

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Diana E. Schlamadinger, Judy E. Kim

¹Chemistry and Biochemistry,University of California San Diego – UCSD

Capítulos

00:05Título
00:44Introduction
01:24Preparation of ~50 nm Diameter Small Unilamellar Vesicles (SUVs) for Membrane Protein Folding
03:10Sample Preparation for Initial Fluorescence Unfolding Curve
04:34Measurement of Fluorescence Spectra
05:45Generation of Initial Unfolding Curve and Estimation of Gibbs Free Energy of Membrane Protein Unfolding
08:21Representative Results for the Measurement of Membrane Protein Unfolding
09:22Conclusion

Summary

Tadução automática

此视频文章详细介绍了实验过程中色氨酸荧光膜蛋白质折叠吉布斯自由能获得。

Tags

Thermodynamics Membrane Protein Folding Fluorescence Spectroscopy Gibbs Free Energy Unfolding Denaturant Integral Membrane Protein E. Coli Equilibrium Populations Folded States Unfolded States Denaturant Concentration Lipid Vesicles Data Analysis Procedure Temperature PH Folding Environments Lipids Micelles

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