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在活动区域的解剖和影像果蝇神经肌肉交界处

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

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在活动区域的解剖和影像果蝇神经肌肉交界处

DOI:

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06:05 min

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April 27, 2011

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Rebecca Smith, J. Paul Taylor

¹Developmental Neurobiology,St. Jude Children’s Research Hospital

Capítulos

00:05Título
01:02Preparation for Dissecting and Immunofluorescent Staining
01:25Dissection of Third-instar Larvae
03:05Removal of Excess Tissue for Immunofluorescence
03:41Mounting of Immunostained Larval Pelts
05:12Larval Mounting and Immunostaining Results
05:50Conclusion

Summary

Tadução automática

神经肌肉接头（NMJ）<em>果蝇</em>是一个重要的模型系统，为研究正常的突触功能以及某些神经系统疾病中发现的突触功能的扰动。我们提出了一个解剖的协议<em>果蝇</em>幼虫电机系统和免疫活性区蛋白内NMJ。

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