/
/
Dissection et l'imagerie de zones actives dans le Drosophile Jonction neuromusculaire
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:02Preparation for Dissecting and Immunofluorescent Staining
- 01:25Dissection of Third-instar Larvae
- 03:05Removal of Excess Tissue for Immunofluorescence
- 03:41Mounting of Immunostained Larval Pelts
- 05:12Larval Mounting and Immunostaining Results
- 05:50Conclusion
La jonction neuromusculaire (JNM) de<em> Drosophila melanogaster</em> Est un système modèle important pour étudier la fonction synaptique normale ainsi que des perturbations de la fonction synaptique dans certaines maladies neurologiques. Nous présentons un protocole de dissection de la<em> Drosophile</em> Larvaires système de moteur et immunocoloration des protéines zone active au sein du MNJ.
Tags
DissectionImagingActive ZonesDrosophila Neuromuscular JunctionSynaptic StructureSynaptic FunctionGenetic ManipulationsLarval Nervous SystemNeurological DiseaseSynaptic MoleculesGlutamatergicActivity-dependent RemodelingIdentified Synaptic TerminalsMotor NeuronsBody-wall Muscle FibersEvolutionarily Conserved Synapse ComponentsMechanisms Underlying Synaptic FunctionMonoclonal AntibodyBruchpilot (Brp)CAST1/ERC Family Member
