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小鼠视网膜神经节细胞的转染在体内电穿孔
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Neurociência
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JoVE Journal Neurociência
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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小鼠视网膜神经节细胞的转染在体内电穿孔

DOI:
10.3791/2678-v

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05:26 min

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April 17, 2011

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,
1Department of Neurobiology,Yale University, 2Program in Developmental Biology,Baylor College of Medicine

Capítulos

  • 00:05Título
  • 01:24Equipment Set-up for in vivo Electroporation
  • 02:08Micropipette and Injector Set-up for Retinal Microinjection
  • 02:44Retinal Injection and Electroporation
  • 04:06Single Retinal Ganglion Cell Labeling with EGFP Plasmid Constructs
  • 05:00Conclusion

Summary

Tadução automática

Tadução automática

我们展示一个<em>在体内</em>电转染视网膜神经节细胞（RGC的）和其他类型的细胞在出生后的小鼠的年龄广泛视网膜的单件或小簇协议。标签和基因操纵产后视网膜神经节细胞的能力<em>在体内</em>是一个发展研究的有力工具。

Tags

TransfectionMouse Retinal Ganglion CellsIn Vivo ElectroporationRGC ProjectionsNeural ConnectivityRetinofugal ProjectionsLateral Geniculate NucleusSuperior ColliculusDevelopmental ChangesAxonal Arbor MorphologyRetinotopic Map FormationGenetic ManipulationElectroporation ProtocolsPostnatal MiceLabeling Efficiency

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