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Capítulos
- 00:06Introduction
- 01:17Device manipulation
- 03:17Preparing the parts for cell seeding
- 05:03Cell seeding
- 07:09Performing the experiment
- 08:26Cleaning chips for reuse
- 13:50Applying polystyrene coating for reuse
- 19:35Discussion
- 21:43Applications
- 22:32Micro-organization: cell membrane contact vs soluble contact
This article describes an experimental approach for dynamic regulation of cell-cell interactions between adherent cells on a micrometer scale. Manipulation of intercellular communication between hepatocytes and stromal cell is demonstrated. The developed platform enables investigation of cell-cell interactions in a variety of biological processes, including development and pathogenesis.
Tags
Silicon MicrochipsCell-cell InteractionCellular MicroenvironmentMalignant TransformationNeighboring StromaConventional TechniquesSpatial And Dynamic ElementsMicromechanical Reconfigurable Culture (RC)Micromachined Silicon SubstrateMechanical RepositioningIntercellular CommunicationCo-cultures Of Hepatocytes And Non-parenchymal CellsSoluble SignalingRC SystemHandling Device PartsActuating Between Gap And Contact ConfigurationsPreparing Substrates For CultureMulti-step Cell Seeding ProcessConfluent Cell MonolayersLive MicroscopyExperimental Configurations
