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一个视神经挤压伤的小鼠模型，研究视网膜神经节细胞生存
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Neurociência
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JoVE Journal Neurociência
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
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一个视神经挤压伤的小鼠模型，研究视网膜神经节细胞生存

DOI:
10.3791/2685-v

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09:07 min

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April 25, 2011

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, , , , , , ,
1National Eye Institute,NIH, 2Ophthalmology Department,The Second Hospital of Harbin Medical University

Capítulos

  • 00:05Título
  • 02:25Baseline Retrograde Labeling of Retinal Ganglion Cells (RGCs) at Day 1
  • 04:47Optic Nerve Crush Injury at Day 4 after Fluorescence Dye Injection
  • 06:22Retinal Harvest and Analysis of RGC Survival
  • 07:13Analysis of Retinal Ganglion Cell Survival after Optic Nerve Crush Injury
  • 08:01Conclusion

Summary

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该协议显示了如何逆行标记视网膜神经节细胞，以及如何随后视神经挤压伤，以分析视网膜神经节细胞的存活和凋亡。这是一个为不同类型的视神经病变，包括青光眼的实验性疾病模型。

Tags

Optic Nerve Crush InjuryRetinal Ganglion Cell SurvivalOptic NeuropathyGlaucomaVision LossIrreversibleExperimental Disease ModelApoptosisNeuronal Death And SurvivalTherapeutic MeasuresTherapeutic ReagentsRetrograde LabelingHarvest The RetinaeRGC Survival

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